New cost-effective method of rapid determination of Mycobacterium tuberculosis drug resistance in Middlebrook semi-liquid medium

Abstract

A comparative evaluation of methods of accelerated drug susceptibility testing of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 system and semi-medium and Middlebrook Soton conducted. 38 panel and47 clinical Mycobacterium tuberculosis strains studied. Parameters testing liquid (BACTEC) and semi Middlebrook media are not differ neither time-bound growth nor in terms of sensitivity and specificity of the method. Semisolid Middlebrook culture allows in a short time (3-4 days) to identify strains of multi-drug resistant, in addition, the method is much cheaper than the BACTEC MGIT 960 SIRE Kit.