Keywords
Abstract
This article represents the results of testing performed to evaluate a new bacterial management technology designed to control the presence of M.tuberculosis in the environment of TB facilities. As part of a study, 137 surfaces were swobbed. It was found that 96.4% of investigated surfaces were contaminated with M.tuberculosis DNA. Door handles had the highest level of M.tuberculosis DNA contamination. Next to it, in descending order, were placed floor surface, furniture and medical equipment, cleaning equipment, hands, clothes and footwear belonging to staff members, shoes and clothing belonging to patients, mobile phones belonging to staff members and hands of patients. M.tuberculosis cultured on digest medium were indicated in 10.9 % of samples. 32.1% samples contained DNA sufficient to detect mutation resistance which is adequate toat least 100-300 M.tuberculosis cells on a swab. Mutation resistance were found in 100% of cases in genes rpoB, katG and gyrA genes, therefore M.tuberculosis DNA isolated from the surface is DNA with multidrug-resistant mutation. Disinfectant “ Lizarin” effectiveness against M.tuberculosis cultures was evaluated. Disinfectant concentration should be increased in 10 times to ensure anti- M.tuberculosis effect, compared with the minimum concentration recommended by the manufacturer. Implementation of new bacterial management technology would allow us to detect the TB activator and/or DNA from object surfaces as well as to establish, within 24 hours, the potential epidemic danger in order to apply the most effective epidemiology measures.